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Effects of a primer terminal 2′-OH group on complementary dNTP binding to wild-type Φ29 DNAP and mutants. The (normalized p/(1 – p) – 1) is shown in a log–log plot as a function of the concentration of dGTP for complexes formed between DNA1-H_H (blue symbols) or DNA1-OH_H (red symbols) with the wild-type (■) or Y254V (▲), D12A/D66A (◆), or D12A/D66A/Y254V (▶) Φ29 DNAP. Error bars show the standard errors.
