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. 2014 Dec 1;53(51):8008–8020. doi: 10.1021/bi501019u

Figure 3.

Figure 3

Analysis of binding of PhoP to the whiB6 promoter sequence containing the direct repeat. (A) Sequence of WB6, the PhoP-binding site on the whiB6 promoter, and its truncated mutants used as competitors in the EMSA. (B) Competition EMSA studies with various truncated WB6 sequences shown in panel A. Biotin-labeled WB6 DNA was incubated without PhoP (lane 1) or with 1 μM PhoP protein in the absence (lane 2) or presence of 4- and 8-fold molar excesses of unlabeled wild-type WB6 (lanes 3 and 4, respectively) and its mutants D1–D7 (lanes 5–18).