Table 1. Thermodynamic Parameters Derived via ITC of Various DNA Sequences with the PhoP Protein.

^aDNA sequences are aligned, except for RD6m29, in which insertion of two nucleotides between nucleotides c and t in the spacer causes a shift of the second motif. A hyphen represents an invariant base, and a delta (Δ) represents a deletion of the nucleotide at that position.

^bN is the stoichiometry, referring to the copy number of DNA per PhoP molecule.

^cValues of TΔS were calculated from the values of K_d and ΔH that were obtained from fitting the ITC titration data with Origin.

^dN.B. stands for no binding under the assay conditions, with a binding dissociation constant of >10 μM.