Figure 1. GPI-0100 significantly enhances HPV16 E7-specific CD8+ T cell responses and antitumor effect induced by TA-CIN in the TC-1 lung metastasis model.

A. Schematic illustration of the experiment design. Briefly, 5∼8 week old female C57BL/6 mice (10 mice/group) were injected with 5×10⁴ TC-1 cells intravenously on day 0. On day 3, the mice were vaccinated with either 6.25 µg/mouse of TA-CIN, or formulated with 50 µg of GPI-0100, or formulated with 50 µg of GPI-0100 in 800 µg of Tween-40 via subcutaneous injection. The mice were boosted twice with the same regimen. On day 27, the mice were sacrificed to harvest lungs and spleens. B. Summary of the number of TC-1 metastasis nodules in the lungs of each mouse (and photographs of the lungs are presented in S2A Fig.). C. Summary of the weight of the lungs. D. Summary of flow cytometry analysis of HPV16 E6 and E7-specific CD8⁺ T cell responses analyzed by IFN-γ intracellular staining. The data were acquired with FACSCalibur, analyzed with CellQuest and representative data is shown in S2B Fig.