Figure 4. Anti-tumor and antigen-specific CD8⁺ T cell responses to TA-CIN/GPI-0100 vaccination in the TC-1 lung metastasis model.

As described in Fig. 3A, 5∼8 weeks old female C57BL/6 mice (10 mice/group) were injected with 5×10⁴ TC-1 cells intravenously on day 0. On day 3, the mice were vaccinated s.c. with either 6.25 µg/mouse of TA-CIN, or formulated with 50 µg of GPI-0100, or formulated with 50 µg of GPI-0100 in 50.7 mg/mL of mannitol and frozen/thawed once. The mice were boosted twice with the same regimen with 1-week interval. On day 21, the mice were sacrificed to harvest lungs and spleens. A. Summary of flow cytometry analysis of HPV16 E6 and E7-specific CD8⁺ T cell responses analyzed by IFN-γ intracellular staining (and representative data is shown in S5B Fig.). B. Summary of the flow cytometry analysis of TA-CIN-specific CD4⁺ T cell responses analyzed by IFN-γ intracellular staining (and representative data is shown in S6A Fig.). C. Summary of the flow cytometry analysis of TA-CIN-specific CD8⁺ T cell responses analyzed by IFN-γ intracellular staining (and representative data is shown in S6B Fig.). The data were acquired with FACSCalibur and analyzed with CellQuest.