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. 2015 Jan 5;10(1):e116389. doi: 10.1371/journal.pone.0116389

Figure 5. Passive transfer of TA-CIN antiserum protects against HPV pseudovirus infection of mouse vagina.

Figure 5

A. Either purified L2-specific neutralizing monoclonal antibody (clone RG-1 at 12.5 25 or 50µg/mouse) or 100 µL pooled immune mouse serum (derived from the study shown in Fig. 2) was passively transferred i.p. into naïve mice. The immune serum used was pooled from groups of 5 mice previously vaccinated three times s.c. at 2 week intervals with either GPI-0100 alone, or 6.25 µg/mouse of TA-CIN alone, or formulated with 50 µg of GPI-0100, or formulated with 50 µg of GPI-0100 in 50.7 mg/mL of mannitol and frozen/thawed once (see schema in Fig. 2A). The mice were challenged intravaginally with HPV16 pseudovirions 24 h after the passive transfer and imaged 3 days later. B. Pre-immune sera (100 µL) or volumes between 1 µL and 100 µL of immune sera after three vaccinations with TA-CIN/GPI-0100 from 3 macaques (I.D 746, 811 or 831) collected in a prior study was passively transferred i.p. into groups of naïve mice (n = 5). The mice were challenged intravaginally with HPV58 pseudovirions 24 h later and imaged 72 hours thereafter to assess infectivity.