Skip to main content
. 2014 Dec 30;10:245–256. doi: 10.2147/IJN.S74868

Figure 5.

Figure 5

Cell viability analysis.

Notes: (A) Viability of SMMC-7221 cells after incubation with different amounts of naked NPs for 48 hours (n=3). The naked NPs did not inhibit the viability of SMMC-7721. (B) Viability of PLC cells after incubation with different amounts of naked NPs for 48 hours (n=3). NPs did not inhibit the viability of PLC. (C) SMMC-7721 cell viability cultured with 5-Fu-loaded NPs at different concentrations of 5-Fu (3.75 μg/mL, 7.5 μg/mL, 15 μg/mL, 22.5 μg/mL, 30 μg/mL) in medium after 48 hours. (D) PLC cell viability of free 5-Fu, 5-Fu loaded IgG-NPs, and 5-Fu-loaded mAb GRP78-NPs at various concentrations of 5-Fu (3.75 μg/mL, 7.5 μg/mL, 15 μg/mL, 22.5 μg/mL, 30 μg/mL) in medium after 48 hours. Data are presented as means ± SD (n=3). Compared with PLC cells, mAb-NPs facilitated the apoptosis of SMMC-7721, which had highly expressed GRP78 at the cell surface.

Abbreviations: 5-Fu, 5-fluorouracil; IgG-NPs, NPs conjugated with IgG; mAb GRP78-NPs, NPs conjugated with monoclonal antibody against GRP78; NPs, nanoparticles; SD, standard deviation.