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. 2013 Dec 2;35(3):294–297. doi: 10.1002/humu.22489

Figure 2.

Figure 2

The impact of the INHA p.Arg60Leu mutation on the amount of secreted inhibins A and B. Activin A and B concentrations in the conditioned media of HEK-293F cells, 2 days after cotransfection of plasmids expressing the wild-type or mutant (p.Arg60Leu) inhibin α-subunit and the wild-type βA- or βB-subunits, were measured using an E4/R1 immunoassay and a C5/R1 immunoassay, respectively. The results correspond to six independent transfections. *, Two-tailed t-test at P < 0.05.