Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 30;10:229–243. doi: 10.2147/IJN.S72264

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Ross et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Expression and characterization of H5₃.

Notes: (A) The HA ectodomain (nucleotides 1–1,723) was amplified from pHW500 and modified by addition of linker sequences, a GCN4pII trimerization domain, and His-tag sequences at the 3′ end. (B) SDS-PAGE and Blue Native gel electrophoresis confirmed the expression of the H5 monomer and trimeric forms. (C) A neutralization inhibition assay demonstrated that the immunogenicity of trimeric H5 was significantly greater than that of monomeric forms, requiring a lower concentration of protein to inhibit 50% of the neutralizing sera (IC₅₀). Error bars represent the standard error of the mean. *Indicates statistical significance. P≤0.0243.

Abbreviations: HA, hemagglutinin; H5, H5 hemagglutinin monomer; H5₃, H5 hemagglutinin trimer; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; IC₅₀, inhibitory concentration 50%.