Figure 6.

Osmotic stress tolerance in wrky54wrky70 was not suppressed by the introduction of sid2-1. (a–c) Three-week-old plants of Arabidopsis wild-type (Col-WT) and sid2-1, wrky54wrky70 and wrky54wrky70sid2-1 mutants were exposed to osmotic stress treatment by watering with 15% polyethylene glycol (PEG)6000 for 3 d. Each genotype was grown in the same pot to perform equivalent treatments. Eight pots were used at the same time and the photographs were taken 1 and 3 d after treatment. Red arrows indicate the wilting symptoms at the tips and edges of the leaves on the first day, with subsequent spread to the whole leaves on the third day. (d) Electrolyte leakage was assessed on leaves after exposure to 15% PEG for 1 and 3 d. Five replicates of each line were used for conductivity measurement. Error bars indicate ± SD from five replicates (*, P < 0.01, one-way ANOVA test).