Table 2.

Effects of Solution Additives on mAb Conformational and Colloidal Stability and Surface Tension

mAb	[NaCl] (mM)	Additive	Tm1, Tm2 (°C)a	kDb	Surface Tension (mN/M)c
mAbA	150	–	67.6, 78.7	−17.4	54.8 ± 0.5
mAbA	0	–	No Tm1, 78.2	−13.1	54.8 ± 0.4
mAbB	150	–	66.5, 83.3	−7.42	55.2 ± 0.2
mAbB	0	–	68.7, 85.4	43.86	54.4 ± 0.4
mAbA	150	0.03% PS80	67.8, 78.6	−16.4	36.7 ± 0.4
mAbA	150	10 mM HPβCD	68.4, 78.9	−20.0	53.5 ± 1.4
mAbA	150	150 mM Arg	66.8, 78.3	−10.3	54.7 ± 0.3
mAbA	150	80 mM Phe	66.2, 77.3	−14.8	45.0 ± 0.2
mAbA	150	80 mM Ile	68.1, 79.0	−14.3	55.3 ± 1.0
mAbA	150	80 mM Leu	68.3, 78.7	−11.0	54.5 ± 0.2

All samples were prepared in 10 mM histidine, pH 6.0.

^aMelting temperatures indicating conformational stability were determined using DSC (n = 2, mean).

^bk_D captures colloidal stability and was determined as the slope/intercept of the D versus [mAb] graph generated from DLS data (n = 3, with six wells measured in each experiment).

^cSurface tensions are reported for 5mg/mL mAb solutions (n = 3, mean ± SE).