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. 2014 Dec 24;9:241–272. doi: 10.2147/DDDT.S71106

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© 2015 Zhang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Effects of Coff/Caff plus Mel on the expression of Wnt3α and β-catenin in N2a/APP cells treated with regimen 1.

Notes: N2a/APP cells were treated with Coff or Caff at 10 μM for 12 hours in the day, followed by Mel at 1, 5, or 10 μM for another 12 hours in the night. Cells were harvested, lysed, and subjected to SDS-PAGE. The target proteins were probed using corresponding primary antibodies. β-actin was used as the internal control for value normalization. (A) Representative blots of Wnt3α and β-catenin; and bar graphs showing the relative expression level of Wnt3α and β-catenin in response to Coff and Mel (B, D), and Caff and Mel (C, E). **P<0.01 and ***P<0.001, by one-way ANOVA.

Abbreviations: ANOVA, analysis of variance; APP, amyloid precursor protein; Caff, caffeine; Coff, coffee; Mel, melatonin; N2a, Neuro-2a; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.