Figure 5.

TCF cells enhance the migration/invasion of breast cancer cells and promote EMT. (A) SCFMs from the indicated cells were added into the lower compartments of 24-well BD BioCoat plates. MCF-7 cells (10⁵) were seeded onto the upper compartment of the plates and incubated for 24 h. The numbers of invaded and migrated cells are presented (mean ± SD; *p < 0.005). (B, C) MCF-7 or normal luminal epithelial cells (NLEC) were treated with the indicated SFCMs for 24 h and cell lysates were used for immunoblotting with the indicated antibodies