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. 2014 Dec 24;10:183–206. doi: 10.2147/IJN.S72580

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© 2015 Bancos et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Confocal evaluation of FITC-E. coli bioparticle internalization.

Notes: (A) RAW 264.7 cells pretreated with SiO₂ NPs (10 nm). Brightness/contrast adjusted 60/50, respectively. Blue is DAPI-stained nuclei and green is FITC-E. coli. (B) Confocal analysis minimizes Au NP quenching effects observed with flow cytometry and (C) allows for semiquantitative analysis of phagocytosis. (D) RAW cells pretreated with Au NPs (300 nm). Brightness/contrast adjusted 60/50, respectively. Blue is DAPI-stained nuclei, green is FITC-E. coli, and red is reflection from Au NPs.

Abbreviations: NPs, nanoparticles; DAPI, 4′,6-diamidino-2-phenylindole; LPS, lipopolysaccharide.