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SIR2 is necessary for the extended RLS of sgf73Δ (A) and ubp8Δ(B), even in strains lacking FOB1 (C, D). E, SIR2OE ubp8Δ double shows extended lifespan comparable to sgf73Δ. Legends show (mean RLS, number of mother cells scored). See also Supplemental Figure S5.
