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Sgf73 interacts with Sir2. (A), Interaction of recombinant Sir2-GST and Sgf73-GFP in the presence and absence of Sir2. Immunoprecipitation was done with glutathione beads coupled to either GST tagged Sir2, GST tagged enzymatically dead Sir2R319K, or GST, and western blot was probed with anti-GFP. (B), Interaction of endogenous Sir2 and Sgf73-GFP. Immunoprecipitation was done using anti-GFP antibody and western blots were probed using anti-Sir2 antibody.
