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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Am J Transplant. 2011 Sep;11(9):1825–1834. doi: 10.1111/j.1600-6143.2011.03730.x

Figure 1. Multiple transfusions with HOD blood do not induce rejection of mOVA BMT or mHA specific CD8+ T cells.

Figure 1

Bone marrow from mOVA mice was stained for OVA expression with polyclonal anti-OVA antiserum and secondary antibody (or secondary along as a control) and evaluated on CD45dim-CD34+ hematopoietic stem cells and CD45hi-CD3+ T cells (B). B6.Thy1.1 mice were given with four weekly transfusions of HOD or control wild-type FVB blood (with no HOD expression) followed by BMT from mOVA donors (Thy1.2). Some mice were infected with PyV or PyV.OVA-I as indicated. Engraftment was assessed 6 weeks after BMT (C). Peripheral blood of animals from each group was stained with anti-CD8 and Kb/SIINFEKL tetramer or Db/LT359 tetramer at the indicated timepoints (D + E). Symbols for panel C, D: FVB ●, HOD ■, PyV + FVB ○, PyV + HOD □, PyV.OVA-I △. Data shown are representative of 3 separate experiments with 2 to 3 mice per group. Error bars represent the standard deviation for the data point.