Skip to main content
. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Am J Transplant. 2011 Sep;11(9):1825–1834. doi: 10.1111/j.1600-6143.2011.03730.x

Figure 3. Transfusion with HOD blood induces expression of IFNγ and TNFα in responding OT-I cells.

Figure 3

IFNγ and TNFα secretion was measured by intracellular cytokine staining after ex vivo peptide restimulation of splenocytes from recipient mice 4 days post transfusion. Representative flow cytometry plots of the staining with or without SIINFEKL peptide restimulation for the different experimental groups are shown. Plots are gated on CD8+ Kb/SIINFEKL tetramer+ events. Combined data shown are the compounded results of 3 separate experiments with 2 mice per group (B). Statistical analysis was performed with two-way ANOVA analysis with Bonferroni post-tests, * = p<0.05, *** = p<0.001. Error bars represent the standard deviation for the data point.