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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Am J Transplant. 2011 Sep;11(9):1825–1834. doi: 10.1111/j.1600-6143.2011.03730.x

Figure 5. Measurement of apoptosis in OT-I T cells stimulated with HOD RBCs.

Figure 5

Apoptosis in live adoptively transferred OT-I cells was measured by staining splenocytes of recipient mice with Kb/SIINFEKL tetramer, 7-AAD, and Annexin V. Representative flow cytometry plots gated on the CD8+ KbSIINFEKL tetramer+ events for the different experimental groups at day 4 after transfusion/infection are shown (A). Data shown are the compounded results of 2 separate experiments with 2 mice per group. Symbols: FVB ●, HOD ■, PyV + FVB ○, PyV + HOD □, PyV.OVA-I △. Error bars represent the standard error of the mean for the data point (B).