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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Bone. 2014 Sep 3;69:12–22. doi: 10.1016/j.bone.2014.08.016

Fig. 1.

Fig. 1

Chondrocyte-specific Cre activity of Col2a1-CreERT2;R26+/+ and chGRKO mice. A, positive X-GAL staining was only detected in the cartilage of Col2a1-CreERT2;R26+/+ mice, and not in other tissues; B, construct of chGRKO mice generated by cross-breeding Col2-CreERT2 mice with GRflox/flox mice. The LoxP sites flanking exon3 of the GR gene resulted in the termination of gene transcription; C, the product of GR knockout was assessed by PCR from multiple tissues including heart, liver, lung, spine, xiphoid and knee joint of 10 + 8 week-old chGRKO mice. Positive expression was detected in spine and long bone, but not in other tissues by PCR using GR1 and GR4 primers. Expression of the glyceraldehyde phosphate dehydrogenase gene (GAPDH) was used as internal control; D, immunohistochemistry staining displayed decreased GR-positive chondrocytes in both the growth plate and articular surface of chGRKO mice compared to CTR mice.