Table 1.

Primers and annealing temperatures used for real-time PCR

Primer	Sequence forward/reverse (5′-3′)	Amplicon size (bp)	Annealing temp (°C)
Pax6	AGGTATTACGAGACTGGCTCC/TCCCGCTTATACTGGGCTATTT	104	60
Atoh1	GAGTGGGCTGAGGTAAAAGAGT/GGTCGGTGCTATCCAGGAG	151	60
Zic1	CAGTATCCCGCGATTGGTGT/GCGAACTGGGGTTGAGCTT	139	60
Zic2	CCGTTCCAGTGTGAGTTTGAG/CTTGCAGAGATAGGGCTTATCG	105	60
Hprt	GGTGAAAAGGACCCCACGA/TCAAGGGCATATCCTACAACA	261	60