Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 5;208(1):23–32. doi: 10.1083/jcb.201409108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Daneshjou et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Rac1 modulates actomyosin-mediated tension across VE-cadherin adhesion. (A) Model of Rac1-mediated inhibition of RhoA–ROCK signaling and mechanical force across VE-cadherin adhesion. (B and C) Cells expressing RhoA biosensor and PA-Rac1. Changes in FRET/CFP ratio after PA-Rac1 activation at time 0 within the indicated region. Ratio images were scaled from 1.5 to 4.5 and color coded as indicated on the right. Representative tracer from reproducible dataset of n = 10. The inset within C is zoomed in on the graph to show the subtle drop in FRET signal. (D) Changes in RhoA activity after photoactivation of PI-Rac1 (−0.03 ± 0.03) or PA-Rac1 (−0.11 ± 0.07); means ± SD, n = 5–10; *, P < 0.05. (E and F) Immunofluorescence staining for myosin light chain phosphorylation (pMLCII) without (dark; E) and with (light; F) Rac1 activation. (G) Quantification of p-MLCII in E and F; means ± SD, n = 11–21; **, P < 0.005; ***, P < 0.0005. (H) Cells expressing VE-cadherin (VE-cad) tension biosensor (VE-t) and PA-Rac1. Control (Ctrl; 1) and PA-Rac1 activation (irradiation; 2) zones are shown enlarged in I. (I) Spatial increase in FRET/CFP ratio was observed at the site of PA-Rac1 activation inside the rectangular region (2) but not the adjacent zone (1). Ratio images were scaled from 0 to 5. (J) Changes in FRET ratio for the tailless (tail) control and VE-t without or with PA-Rac1 activation; means ± SD, n = 7–10; **, P < 0.005. Times are given in minutes and seconds. Bars: (B [main image], E, H, and I) 10 µm; (B [insets]) 5 µm.