FIG 4.

Living Trichobilharzia regenti cercaria in vitro, shedding its glycocalyx upon stimulation by linoleate. The sticky products of the penetration glands, stained with lithium carmine, adhere to the surface of the body, and as the cercaria crawls forward by periodical constrictions (A) and extensions (B), the glycocalyx and bound secretions are removed from the surface in a sleeve-like manner. Arrows indicate detached sleeve-like remnants of glycocalyx and gland products. Bar, 100 μm. (Courtesy of J. Chaloupecká, reproduced with permission.)