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. 2015 Jan;28(1):54–79. doi: 10.1128/CMR.00028-14

TABLE 1.

Performance of culture methods and nucleic acid amplification assays for detecting Kingella kingae in children with skeletal system infectionsa

Yr Reference Culture method No. with positive K. kingae culture/total PCR method Target gene
No. with positive PCR/total
C-PCR RT-PCR
1998 301 C 0/1 C 16S rRNA gene NA 1/1
2003 186 C + BCV 13/18 C 16S rRNA gene NA 18/18
2005 303 C + BCV 9/24 C 16S rRNA gene NA 24/24
2007 302 C + BCV 6/21 C + RT 16S rRNA gene 16S rRNA gene 20/21
2007 16 C + BCV 17/39 C + RT 16S rRNA gene cpn60 39/39
2008 200 BCV 2/4 C + RT 16S rRNA gene cpn60 4/4
2008 206 C 0/1 C 16S rRNA gene NA 1/1
2009 15 C + BCV 7/31 RT ND cpn60 31/31
2009 88 C 0/2 C + RT 16S rRNA gene rtxA + rtxB 2/2
2009 158 C + BCV 5/18 RT ND ? 18/18
2010 89 C 0/23 RT ND rtxA + rtxB 23/23
2011 90 ND 2/20 RT ND rtxA 20/20
2013 151 C + BCV 5/44 RT ND cpn60 43/44
2013 152 C ? C + RT 16S rRNA gene cpn60 11/11
2014 160 C + BCV 2/24 C + RT ND rtxA 27/27
2014 304 C 0/3 RT ND cpn60 3/3
2014 305 C + BCV 0/1 RT ND cpn60 1/1
Total 68/274 (24.8%) 388/390 (99.5%)
a

Abbreviations: C, conventional; BCV, blood culture vial; RT, real time; ND, not done; NA, not applicable.