TABLE 1.
Yr | Reference | Culture method | No. with positive K. kingae culture/total | PCR method | Target gene |
No. with positive PCR/total | |
---|---|---|---|---|---|---|---|
C-PCR | RT-PCR | ||||||
1998 | 301 | C | 0/1 | C | 16S rRNA gene | NA | 1/1 |
2003 | 186 | C + BCV | 13/18 | C | 16S rRNA gene | NA | 18/18 |
2005 | 303 | C + BCV | 9/24 | C | 16S rRNA gene | NA | 24/24 |
2007 | 302 | C + BCV | 6/21 | C + RT | 16S rRNA gene | 16S rRNA gene | 20/21 |
2007 | 16 | C + BCV | 17/39 | C + RT | 16S rRNA gene | cpn60 | 39/39 |
2008 | 200 | BCV | 2/4 | C + RT | 16S rRNA gene | cpn60 | 4/4 |
2008 | 206 | C | 0/1 | C | 16S rRNA gene | NA | 1/1 |
2009 | 15 | C + BCV | 7/31 | RT | ND | cpn60 | 31/31 |
2009 | 88 | C | 0/2 | C + RT | 16S rRNA gene | rtxA + rtxB | 2/2 |
2009 | 158 | C + BCV | 5/18 | RT | ND | ? | 18/18 |
2010 | 89 | C | 0/23 | RT | ND | rtxA + rtxB | 23/23 |
2011 | 90 | ND | 2/20 | RT | ND | rtxA | 20/20 |
2013 | 151 | C + BCV | 5/44 | RT | ND | cpn60 | 43/44 |
2013 | 152 | C | ? | C + RT | 16S rRNA gene | cpn60 | 11/11 |
2014 | 160 | C + BCV | 2/24 | C + RT | ND | rtxA | 27/27 |
2014 | 304 | C | 0/3 | RT | ND | cpn60 | 3/3 |
2014 | 305 | C + BCV | 0/1 | RT | ND | cpn60 | 1/1 |
Total | 68/274 (24.8%) | 388/390 (99.5%) |
Abbreviations: C, conventional; BCV, blood culture vial; RT, real time; ND, not done; NA, not applicable.