FIG 3.

Pairwise distance distribution histograms of 59 representative sapovirus complete VP1 sequences. The vertical dashed lines indicate the cutoff limits for genogrouping and/or genotyping. (A) Limits based on nucleotide sequences, with three peaks (0 to 0.159, 0.198 to 0.471, and 0.522 to 0.807) corresponding to the strain, genotype, and genogroup range, respectively. The mean values ± 3 SD for the pairwise distance peaks were 0 to 0.151, 0.170 to 0.416, and 0.489 to 0.801, and the cutoff values for the genotype and genogroup clusters were designated ≤0.169 and ≤0.488, respectively. (B) Limits based on amino acid sequences, with two major peaks (0 to 0.480 and 0.652 to 1.115) corresponding to the strain and genogroup range, respectively.