Fig. 1.

Wound healing induced by low level light therapy (LLLT) and NO-Cbi. (a and b) U2OS cells received a mechanical scratch wound followed by treatment with LLLT, $5\mu \mathrm{M}$ NO-Cbi, or the combination of LLLT and NO-Cbi. Wound recovery was followed for a period of 24 h at which time the final wound area was determined and normalized to the initial wound size. The images being shown are at 24-h post-treatment. The normalized average wound area was determined by dividing the final wound area by the initial quantified wound area and averaging the replicates. The green area marks the wound. Bar, $100\mu \mathrm{m}$. (c) The MTS proliferation assay showed no statistically significant differences between the untreated control. Data represent the $\text{mean}\pm \text{standard error}$ of the mean (SEM) of at least three separate experiments with $n=12$ (a and b) or $n=6$ for (c) per experiment. ****$P<0.0001$, ***$P<0.001$, and **$P<0.01$ compared to untreated control or indicated comparison.