Fig. 4.

Western blot and migration analysis of phospho-ERK1/2. (a) U2OS cells were treated with NO-Cbi, LLLT, or a combination of LLLT and NO-Cbi. ERK1/2 phosphorylation and total ERK1/2 expression were determined by western blot analysis (left). The intensity of pERK1/2 expression was quantified and then normalized to the total ERK1/2 expression and the untreated control (right). (b) A mechanical scratch wound was generated in U2OS cells, and the cells were treated with $50\text{-}\mu \mathrm{M}$ sodium azide, a C-ox inhibitor and $5\text{-}\mu \mathrm{M}$ U0126, an MEK-specific inhibitor. The wound closure rate was quantified after 24 h. Data are representative of at least three separate experiments for (a) and data represent the $\text{mean}\pm \mathrm{SEM}$ of at least three separate experiments with $n=12$ per experiment for (b). ****$P<0.0001$ and *$P<0.05$ compared to untreated control.