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. 2014 Aug 16;60(6):406–410. doi: 10.1262/jrd.2013-108

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©2014 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 1. — Analysis of semen samples of male germline chimeras produced by transfer of female and male gonadal germ cells collected from 7-day-old embryonic gonads. DNA was extracted from the sperm samples and analyzed for the presence of (A) dominant white-specific repeat sequences by PCR with dominant white-specific primers and (B) recessive allele-specific repeat sequences by PCR with recessive allele-specific primers. Lane 1, control (White Leghorn sperm DNA); lane 2, control (Rhode Island Red sperm DNA); lane 3, negative control (water); lane 4, ID no. F→M2 (male germline chimera produced by transfer of female gonadal germ cells); lane 5, ID no. M→M3; lane 6, ID no. F→M6; lane 7, ID no. M→M7; lane 8, ID no. M→M8.