Skip to main content

View full-text article in PMC

. 2014 Sep 15;60(6):438–446. doi: 10.1262/jrd.2014-013

Table 1. Primers used for qPCR of mink uterine gene transcripts and amplicon characteristics.

Genes	Forward & reverse primers	Amplicon	Melt temp. (C)	Accession No.*
G6pc3	ATCAGCCTAGCCTTCAAGTGGTGT	187 bp	87.78 ± 0.22	NM_138387.3
G6pc3	AGG CTATCTTCTGGCCATTTCCCA	187 bp	87.78 ± 0.22	NM_138387.3

Gapdh	TTGTCAGCAATGCCTCCTGTACCA	200 bp	85.03 ± 0.48	AF076283.1
Gapdh	ACCAGTGGAAGCAGGGATGATGTT	200 bp	85.03 ± 0.48	AF076283.1

Actb	GATGACC CAGATCATGTTCGAG	321 bp	86.0 ± 0.03	AF076283.1
Actb	CCATCTCCTGCTCGAAGTCC	321 bp	86.0 ± 0.03	AF076283.1

Glucose-6-phosphatase-3 (G6pc3); Glyceraldehyde 3-phosphate dehydrogenase (Gapdh); beta-actin (Actb). *http:/www.ncbi.nlm.nih.gov.