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Isolated pre-antral follicles from vitrified-warmed cortical tissue from a 31-year-old woman. (a) A 37-μm-diameter follicle (primordial), (b) a 52-μm-diameter follicle (primary) and (c) a 71-μm-diameter follicle (secondary) were isolated from vitrified-warmed cortical tissue digested with collagenase I and subsequently trimmed by using 31G needles.
