Fig. 2.

LITAF 192 V causes changes in the amount of PMP22 protein. BGMK cells were transiently transfected with a, b FLAG-tagged PMP22 and b myc-tagged I92V. LysoTracker or MitoTracker was applied to live cells 24 h post-infection, and cells were then fixed and permeabilized. Cells then underwent indirect immunofluorescence, and I92V was detected using anti-myc antibodies, and PMP22 was visualized using anti-FLAG antibodies. Nuclei were visualized using differential interference contract (DIC). All images were taken using a laser scanning confocal microscope