Fig. 1.

Infection with IAV reduces the expression of β-catenin in adherens junctional proteins in HUVECs. A. Representative immunoblot (from three separate experiments) of β-catenin and VE-cadherin in cell lysates (20 μg protein/lane) of HUVECs after infection for 28 h with or without IAV PR8. As a control, saline was used as no-infection agent. B. Relative levels of β-catenin and VE-cadherin in the blot in panel A (n = 3), determined as described in Materials and methods. Statistical analysis was performed using the unpaired t-test. C. Representative RT-PCR data of β-catenin in control (uninfected) and IAV-infected HUVECs at 28 h post-transfection. D: Relative levels of β-catenin in the RT-PCR experiment shown in panel C (n = 3). Statistical analyses were conducted using the unpaired t-test