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Antibodies used for immunohistochemistry of pulmonary metastases
| Primary antibody |
Secondary antibody |
|||
|---|---|---|---|---|
| Targeted antigen | Dilution | Company | Source | Dilution |
| Vimentin | 1:100 | DakoCytomation | Biotinylated Horse Anti-Mouse | 1:200 |
| B220 | 1:200 | BD Biosciences | Biotinylated Rabbit Anti-Rat | 1:100 |
| CD3 | 1:1,000 | DakoCytomation | Biotinylated Goat Anti-Rabbit | 1:1,000 |
| F4/80 | 1:100 | AbD Serotec | Anti-Rat | 1:200 |
| CD45 | 1:500 | BD Biosciences | Anti-Rat | 1:100 |
