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. 2004 Jun 7;101(24):8906–8911. doi: 10.1073/pnas.0403000101

Fig. 2.

Fig. 2.

RNAi depletion of DmBLM and DmKu affects P element excision and repair activity. (A) P element excision and repair activity. The percent of the control excision and repair activity was calculated within each assay, and activities from at least three different assays were averaged with the standard deviations indicated. (B) Immunoblot of DmBLM and DmKu RNAi-treated Drosophila L2 cells. Immunoblot analysis of RNAi-treated samples after transfection shows specific depletions in the protein level of DmBLM, DmKu70, and DmKu80. DmBLM RNAi decreases the DmBLM protein level (lanes 2, 5, 6, and 8). Depletion of one DmKu subunit results in the decrease in the other as shown in lanes 3 and 4. U2AF50 antibody was used as a control (30). imagequant software was used to quantitate the signals for DmKu relative to the U2AF50 loading control signal.