Fig. 4.

The ERRα inverse agonist XCT790 inhibits PGC-1α regulation of ERRα and ERRα target gene expression. (A) Transient transfection in CV-1 cells, demonstrating ERRα and PGC-1α activation of the human MAOB promoter reporter. At 6 h after transfection, cells were treated for 18 h with the indicated concentrations of DMSO or XCT790. (B–D) Quantitative RT-PCR of ERRα (B), MAOB (C), and medium-chain acyl CoA dehydrogenase (MCAD) (D). mRNA expression in MDA-MB-231 cells infected with a control retrovirus or a PGC-1α retrovirus for 24 h, followed by treatment with 10 μM XCT790 for 4 days before RNA analysis, is shown. mRNA levels are expressed relative to the amount of cyclophilin mRNA. RLU, relative light units.