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. 2015 Jan 6;5:7633. doi: 10.1038/srep07633

Figure 4. Antagonism of PPARγ prevents high-fat-diet-induced obesity in vivo.

Figure 4

High-fat-diet (HFD) chowed C57/BL6 mice were subjected to GW9662 at a dose of 0.35 mg/kg per day in their drinking water for 5 weeks (GW9662 group, n = 8) or to a corresponding volume of vehicle (control group, n = 8). (A) Increases in body fat were suppressed by GW9662 treatment, and the adipose mass of Epi fat, retroperitoneal fat (RP fat) and adipocyte size were diminished in the GW9662 group. (B) Epi-WAT weight, WAT index, weight at 18 weeks and weight changes were measured. #p < 0.05 compared with control group. (C) The protein expression of GPAT3 was measured using western blots. The gels were run under the same experimental conditions, and cropped blots are used here. The full-length gel images are available in Supplementary Fig. 6. (D) Intracellular TG levels and plasma adiponectin were detected, respectively. The results are expressed as the mean ± SD. #p < 0.05 compared with control group. All of the above panels are representative of 3 independent experiments.