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. 2015 Jan 6;5:7633. doi: 10.1038/srep07633

Figure 6. The effects of TSH on adipogenesis were reversed when the expression of Tshr was silenced in vivo and in vitro.

Figure 6

(A–C) Differentiated 3T3-L1 cells were transfected with non-targeting or Tshr siRNA. After 48 h, the cells were incubated with or without TSH (2 μM), for 24 h. (A) TG contents and (B) lipid contents were measured, respectively. #p < 0.05 compared with non-targeting siRNA group; ★p < 0.05 compared with TSH group. (C) The p-AMPK, total AMPK, GPAT3 and PPARγ protein expression were analyzed by western blot. (D–H) Tshr−/− (n = 8) and Tshr+/+ (n = 8) mice were fed a high-fat diet for 10 weeks. (D) Weight gain was decreased in Tshr−/− mice. Body weight was monitored twice a week. (E) Adipose mass of Epi fat, RP fat and the size of the adipocytes were diminished in the Tshr−/− group. (F) Epi-WAT weight and the WAT index were assayed. #p < 0.05 compared with the Tshr+/+ group. (G) The protein expression levels of GPAT3 were detected by western blot. (H) Intracellular TG levels and plasma adiponectin were detected. The results are expressed as the mean ± SD. #p < 0.05 compared with Tshr+/+ group. All of the above panels are representative of 3 independent experiments. All the gels were run under the same experimental conditions.