Figure 6. Klotho is critical for Tet1-mediated neuronal protection in response to oxidative stress.

(a) CGNs were transfected with either Klotho shRNA plasmid or HA-tagged Tet1-CD and both plasmids, treated with H₂O₂ and immunostained for HA, GFP and Hoechst for examining neuronal cell death. Yellow arrowhead stands for the healthy cells and red arrowhead indicates apoptotic cells. (b) Quantitation of (a). (ANOVA followed by Tukey's test, ***p < 0.001, **p < 0.01, n = 4). Error bar represents s.e.m. Scale bar = 50 μm.