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. 2014 Dec 17;111(52):18590–18595. doi: 10.1073/pnas.1415365112

Fig. 6.

Fig. 6.

RS1 enhances the effect of a PDK1 active site inhibitor to more effectively block activation of both S6K1 and AKT. (A) Effect of RS1 on the dose-dependent inhibition of S6 phosphorylation by the PDK1 inhibitor GSK2334470. Cell lysates were immunoblotted with antibodies for phospho-S6 S235/6 and α-tubulin. (B) Effect of RS1 on the dose-dependent inhibition of AKT phosphorylation by the PDK1 inhibitor GSK2334470. The experiment was the same as that depicted in A, except cells were immunoblotted with antibodies for phospho-AKT T308 and α-tubulin. The level of phosphorylation was quantified from the infrared signal and normalized to the α-tubulin signal. Scatterplots represent data pooled from five independent experiments. Statistical significance was calculated using an unpaired two-sided t test. *P < 0.05; **P < 0.01; ***P < 0.001.