Fig. 6.

SCUBA analysis of the single-cell RNA-seq data in mouse embryo. (A) Lineage tree reconstructed by SCUBA. Node sizes are proportional to number of cells. (B) Comparison of the 32-cell bifurcation directions derived from the RNA-seq vs. RT-PCR datasets. Scatter plot shows the gene weights associated with each of the 13 common genes between the two datasets. (C) Distribution of gene weights for the 1,000 most variable genes. Some well-characterized regulators are indicated. (D) Equilibrium distribution and (E) potential function V(x) corresponding to the 32-cell bifurcation.