Fig. 3.

ft-1 mutant seeds are affected in seed procyanidin content and seed coat permeability. (A) Insoluble (solid bars) and soluble (open bars) procyanidin content measured by acid butanol extraction and absorbance at 550 nm in WT, ft-1, and gi-3 mutant seeds from plants that had experienced the indicated temperature regime before flowering, but all maintained after flowering at 22 °C. Data represent mean and SE of five biological replicate seed batches, with the exception of gi-3, for which only three were available. Astersisks denote significant differences (P < 0.01). (B) Tetrazolium uptake of WT and ft seeds after 48 h from plants raised at 22 °C before fertilization of the first ovules, compared with wild-type seeds from plants that experienced 16 °C. tt4-1 mutants are shown as positive control. All plants were maintained at 22 °C after bolting. Pictures of three biological replicate seed batches are shown.