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. 2014 Dec 15;111(52):18631–18636. doi: 10.1073/pnas.1422488112

Fig. 2.

Fig. 2.

NBS1 nucleolar localization after DNA damage. (A) Representative pictures of U2OS cells expressing HA-NBS1 stained with antibodies against the HA tag (green) or TCOF1 (red) with or without IR treatment (10 Gy). Images of cells with merged green and red signals are indicated. (B) Quantification of the percentage of U2OS cells expressing HA-NBS1 that exhibit nucleolar staining of NBS1 with or without IR treatment (10 Gy) as in A. (C) Representative pictures of U2OS cells expressing HA-NBS1 stained as in A treated with control or TCOF1 siRNAs with IR treatment (10 Gy). Images of cells with merged green and red signals are indicated. (D) Western blotting showing the levels of TCOF1 knockdown obtained with two TCOF1 siRNAs. (E) Quantification of the percentage of U2OS cells expressing HA-NBS1 that exhibit nucleolar staining of NBS1 after treatment with TCOF1 siRNAs and IR.