Fig. 3.
NBS1 localization to nucleoli after CK2 depletion or ATM inhibition. (A) Representative images of U2OS cells expressing HA-NBS1 treated with control or siRNAs targeting the CK2 subunits α and α′ (CK2α and CK2α′) and stained as in Fig. 2 after IR treatment (10 Gy). (B) Quantification of the percentage of U2OS cells expressing HA-NBS1 that exhibit nucleolar staining treated as in A. (C) Representative images of U2OS cells expressing HA-NBS1 with or without ATM inhibitor (10 μM) and stained as in Fig. 2 after IR treatment (10 Gy). (D) Quantification of the percentage of U2OS cells expressing HA-NBS1 that exhibit nucleolar staining treated as in C.