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. 2014 Dec 19;111(52):18673–18678. doi: 10.1073/pnas.1413687112

Fig. 2.

Fig. 2.

Specific inhibition of IL-17A secretion from TcR-stimulated splenocytes by tRORγt-TMD. (A–C) tRORγt-TMD did not affect the induced expression of CD69 (A) and FasL (B) on mouse splenocytes, and IL-2 secretion (C) from the splenocytes activated with plate-bound anti-CD3 and soluble anti-CD28 antibodies. The cells were stained with anti-CD69 or anti-FasL mAb and analyzed by FACS, and the level of IL-2 in the culture media was analyzed by ELISA. (D–F) tRORγt-TMD did not affect cytokine production from either TH1 (IFN-γ) (D) or TH2 (IL-4) (E) cells. (F) Dose-dependent inhibition of IL-17A production from TcR-stimulated splenocytes by tRORγt-TMD. The levels of IFN-γ, IL-4, or IL-17A in the culture media were analyzed by ELISA. Data are representative of at least three independent experiments. Error bars denote SEM. **P < 0.01.