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. 2014 Dec 15;111(52):18584–18589. doi: 10.1073/pnas.1413282112

Fig. 3.

Fig. 3.

Cleavage of VWF by ADAMTS13 under fluid shear stress. VWF cleavage products were detected by SDS/PAGE and immunoblotting with anti-VWF. The arrowheads indicate the 350-kDa VWF fragment produced by ADAMTS13. Plasma VWF (40 nM) was sheared for the indicated times (seconds) with (A) MDTCS or ADAMTS13 (50 nM), or (B) with ADAMTS13 (50 nM) without or with monoclonal antibody 12D4 (40 µg/mL). (C) Densitometric analysis to compare the rate of VWF cleavage by ADAMTS13 without (orange squares) or with (green triangles) monoclonal antibody 12D4, or by MDTCS (blue circles). Values are normalized to cleavage at 300 s by ADAMTS13 alone and plotted as the mean ± SE for two experiments. (D) Duplicate reactions with ADAMTS13 were sheared for 300 s without or with monoclonal antibody 19H4 (100 µg/mL) or 12D4 (40 µg/mL). Control reactions were sheared for 300 s in the presence of 10 mM EDTA.