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Purification of trophoblast cells. Both normal and GDM trophoblast were stained with these Abs followed by Alexa Fluor 488 anti-mouse as secondary Ab. Negative control represents trophoblast without CK-7 stain. In normal placenta the trophoblast of 82 ± 0.91% appeared as positive and in GDM placenta 86.55 ± 5.07% of trophoblast were stained with CK7. The cell lines stained with NDOG1 appeared as 56.2 ± 1.03% positive stain and 49.7 ± 2.37% pure for GDM trophoblast.
