Fig. 7.

Impact of tunicamycin on the 5-HT uptake rates of trophoblast. The 5-HT uptake rates of intact trophoblast cells were measured following pretreatment with tunicamycin at various concentrations (44). [³H]-5HT uptake rates were measured in intact cells (2.3 × 10⁵ per assay) (37, 39). Rate of uptake is expressed as the means and SD values of triplicate determinations from three independent samples in each group. Asterisks indicate statistical difference between normal and GDM trophoblast (*); treated and untreated trophoblast (**). All assays were performed in triplicate.