Fig. 9.

The physical association between SERT and ERp44 in trophoblast. The lysates of trophoblast (1.5 × 10⁶ per assay) were prepared and subjected to IP in the presence (+Ab) or absence (−Ab) of monoclonal SERT Ab (A) or polyclonal ERp44 Ab (39) (B). The blots show the level of association between SERT-ERp44 elevated in GDM trophoblast. To verify these findings SERT Ab depleted lysates were analyzed for the level of unbound ERp44 in both groups (C). The band densities were calculated as the ratio of each band to the level of actin and the SERT levels are expressed as the means and SD values of triplicate determinations from three independent experiments; all groups, n = 5. Averaged data from three independent experiments are presented ± SE. The values are statistically different (P < 0.001, Student t test).