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. 2014 Jun 4;1(6):446–456. doi: 10.18632/oncoscience.49

Figure 3. Treatment with AICAR or A-769662 induced G2/M arrest in PC3 and PC3M cells and cyclin B1 accumulation.

Figure 3

(A, B, E, F) Cell-cycle profile analysis in (A, E) PC3 and (B, F) PC3M cells treated with (A, B) AICAR (500 and 2000 μM) or (E, F) A-769662 (50 and 100 μM) in the presence or absence of Compound C (10 μM). Data of cell-cycle profile analysis were presented as mean ± SD. * indicates significant difference at P ≤ 0.001, # at P ≤ 0.005 and ▲ at P ≤ 0.01. (C, D, G, H) Western blot of whole cell lysates from (C, G) PC3 and (D, H) PC3M cells treated with (C, D) AICAR (500 and 2000 μM) for 2 h and 24 h or (G, H) A-769662 (50 and 100 μM) for 1 h, probed for cyclin B1 protein. Values under Western blots represent level of each protein normalized to GAPDH.