Table 1. The percentage of larvae that showed lower serotonin signals in SE0_PG neurons.

GFP	Serotonin	96 hAH	120 hAH
−	−	30 (n=9)	17.9 (n=5)
+	−	10 (n=3)	82.1 (n=23)
+	+	60 (n=18)	0 (n=0)
	%	100	100

GFP, green fluorescent protein; hAH, hours after hatching; MARCM, mosaic analysis with a repressible cell marker; PG, prothoracic gland; rpr, reaper.

The ‘GFP[–] and serotonin[−]’ group indicates that SE0_PG neurons were lost by rpr. The ‘GFP[+] and serotonin[−]’ group indicates that SE0_PG neurons were present but the serotonin signal decreased. The ‘GFP[+] and serotonin[+]’ group indicates that SE0_PG neurons and the serotonin signal were detected on the PG. When the MARCM clone-induced larvae were dissected at 96 hAH (the wandering stage), 40% of these animals showed decreased level of serotonin in SE0_PG neurons. In contrast, when the larvae delayed for pupariation were dissected at 120 hAH, all animals decreased serotonin signals in SE0_PG neurons. Thus, the inhibition of SE0_PG neurons leads to decreased level of serotonin, which correlates with the delayed timing of pupariation.